Radioimmunoassay for the Middle Region of Human Parathyroid Hormone: Studies with a Radioiodinated Synthetic Peptide*
作者： STEPHEN J. MARX , MARK E. SHARP , ADRIAN KRUDY , MICHAEL ROSENBLATT , LAWRENCE MALLETTE
DOI: 10.1210/JCEM-53-1-76
关键词: Chemistry 、 Radioimmunoassay 、 Radioligand 、 Hormone 、 Endocrinology 、 Primary hyperparathyroidism 、 Hyperparathyroidism 、 Internal medicine 、 Albumin 、 Parathyroid gland 、 Teriparatide
摘要: We radioiodinated a synthetic fragment representing residues 44-68 from the middle region of human parathyroid hormone (hPTH). At least 90% purified [1125I]- hPTH-(44–68) was able to bind anti-hPTH serum. Antibodybound [l25I]hPTH-(44–68) could be rapidly and efficiently separated nonbound radioligand by dextran-coated charcoal. [125I]hPTH-(44–68) not degraded after 72-h incubation in undiluted plasma at 7 C, it stable for many weeks -20 C 1% albumin buffer. [l25JhPTH-(44–68) used develop midregion specific PTH RIAs. The immunoreactive concentration above upper limit normal range 39 43 patients with primary hyperparathyroidism. Values assay an established carboxy-terminus correlated using peripheral 17 hyperparathyroidism (r = 0.84; P < 0.0001) or gland venous effluent same 0.79; 0.0005). Gel filtration analysis ...
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