Effects of Scriptaid on Cell Cycle and Histone Acetylation of Ovine Nuclear Donor Cumulus Cells and their Ability to Support the Development of Somatic Cell Nuclear Transfer Embryos
作者： Bingqiang Wen , Shuchun Sun , Junjie Li , Shujun Tian , Lu Li
DOI:
关键词: Histone deacetylase inhibitor 、 Acetylation 、 Blastocyst 、 Molecular biology 、 Histone H4 、 Histone 、 Biology 、 Somatic cell nuclear transfer 、 Histone H3 、 Cell cycle 、 Andrology
摘要: Compelling evidence suggests that histone deacetylase inhibitor (HDACi) influences the development of somatic cell nuclear transfer (SCNT) embryos. The current study was conducted to determine effect pretreatment donor cumulus cells with Scriptaid (a novel HDACi) on cycle, acetylation and cloning embryos in ovine. First, we optimized efficiency a dose (0, 0.1, 0.2, 0.4 0.8 μmol/L) time-dependent 12, 24, 36, 48 h) manner developmental capacity these Then, quantitatively assessed alterations levels H3 lysine 9 (acH3K9) H4 12 (acH4K12) SCNT by immunofluorescence staining. Furthermore, detected proportion G0/G1 phase cells. We found significantly improved blastocyst rates derived from pretreated mild (0.2 for 24 hours (21/86 [24.39%] vs. 11/85 [12.91%]; P<0.05). Meanwhile, acH3K9 acH4K12 were also (P<0.05). Moreover, more compared control group (84.22% 75.96%, In conclusion, treated is beneficial early embryos, ascending acH3K9/ cell. can be used improve
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