Effect of synchronization of donor cells in early G1-phase using shake-off method on developmental potential of somatic cell nuclear transfer embryos in cattle.
作者： Yuji Goto , Muneyuki Hirayama , Kazuya Takeda , Nobuyuki Tukamoto , Osamu Sakata
DOI: 10.1111/ASJ.12047
关键词:
摘要: In this study, we compared the developmental ability of somatic cell nuclear transfer (SCNT) embryos reconstructed with three bovine cells that had been synchronized in G0-phase (G0-SCNT group) or early G1-phase (eG1-SCNT group). Furthermore, investigated production efficiency cloned offspring for NT derived from these donor cells. The and eG1-phase were synchronized, respectively, using serum starvation antimitotic reagent treatment combined shaking plate containing (shake-off method). fusion rate G0-SCNT groups (64.2 ± 1.8%) was significantly higher than eG1-SCNT (39.2 1.9%) (P < 0.05), but rates to blastocyst stage SCNT per fused oocytes similar all groups. overall clone (12.7%) recipient cow (3%) 0.05). mean birth weight calves average calving score (48.1 3.4 kg 3.3 0.3, respectively) 0.05) those (37.2 2.1 2.3 0.2, respectively). Results study indicate synchronization shake-off method improved transferred embryo.
sci-hub.se 参考文章(35)
Daisaku Iwamoto, Aya Kasamatsu, Atsushi Ideta, Manami Urakawa, Kazuya Matsumoto, Yoshihiko Hosoi, Akira Iritani, Yoshito Aoyagi, Kazuhiro Saeki, Donor cells at the G1 phase enhance homogeneous gene expression among blastomeres in bovine somatic cell nuclear transfer embryos. Cellular Reprogramming. ,vol. 14, pp. 20- 28 ,(2012) , 10.1089/CELL.2011.0035
O. Hayes, B. Ramos, L.L. Rodríguez, A. Aguilar, T. Badía, F.O. Castro, Cell confluency is as efficient as serum starvation for inducing arrest in the G0/G1 phase of the cell cycle in granulosa and fibroblast cells of cattle. Animal Reproduction Science. ,vol. 87, pp. 181- 192 ,(2005) , 10.1016/J.ANIREPROSCI.2004.11.011
I. Wilmut, A. E. Schnieke, J. McWhir, A. J. Kind, K. H. S. Campbell, Viable offspring derived from fetal and adult mammalian cells Nature. ,vol. 385, pp. 810- 813 ,(1997) , 10.1038/385810A0
W. M. Rideout III, Nuclear Cloning and Epigenetic Reprogramming of the Genome Science. ,vol. 293, pp. 1093- 1098 ,(2001) , 10.1126/SCIENCE.1063206
B.P. Enright, L.-Y. Sung, C.-C. Chang, X. Yang, X.C. Tian, Methylation and Acetylation Characteristics of Cloned Bovine Embryos from Donor Cells Treated with 5-aza-2′-Deoxycytidine Biology of Reproduction. ,vol. 72, pp. 944- 948 ,(2005) , 10.1095/BIOLREPROD.104.033225
D.N Wells, G Laible, F.C Tucker, A.L Miller, J.E Oliver, T Xiang, J.T Forsyth, M.C Berg, K Cockrem, P.J L’Huillier, H.R Tervit, B Oback, Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle. Theriogenology. ,vol. 59, pp. 45- 59 ,(2003) , 10.1016/S0093-691X(02)01273-6
Jose B Cibelli, Steve L Stice, Paul J Golueke, Jeff J Kane, Joseph Jerry, Cathy Blackwell, F Abel Ponce de León, James M Robl, Cloned Transgenic Calves Produced from Nonquiescent Fetal Fibroblasts Science. ,vol. 280, pp. 1256- 1258 ,(1998) , 10.1126/SCIENCE.280.5367.1256
Chang-Kyu Lee, Jorge A. Piedrahita, Inhibition of apoptosis in serum starved porcine embryonic fibroblasts Molecular Reproduction and Development. ,vol. 62, pp. 106- 112 ,(2002) , 10.1002/MRD.10058
Karen L. Jones, Jonathon Hill, Tae Young Shin, Ling Lui, Mark Westhusin, DNA hypomethylation of karyoplasts for bovine nuclear transplantation. Molecular Reproduction and Development. ,vol. 60, pp. 208- 213 ,(2001) , 10.1002/MRD.1079
Atsushi Ideta, Koh Hayama, Manami Urakawa, Kanami Tsuchiya, Yoshito Aoyagi, Kazuhiro Saeki, Comparison of early development in utero of cloned fetuses derived from bovine fetal fibroblasts at the G1 and G0/G1 phases Animal Reproduction Science. ,vol. 119, pp. 191- 197 ,(2010) , 10.1016/J.ANIREPROSCI.2010.02.006