Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle.
作者： D.N Wells , G Laible , F.C Tucker , A.L Miller , J.E Oliver
DOI: 10.1016/S0093-691X(02)01273-6
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摘要: Abstract Several studies have shown that both quiescent and proliferating somatic donor cells can be fully reprogrammed after nuclear transfer (NT) result in viable offspring. So far, however, no comparative study has conclusively demonstrated the relative importance of cell cycle stage on cloning efficiency. Here, we compare two different types bovine fetal fibroblasts (BFFs) were synchronized G 0 , 1 phases within . We show for non-transgenic (non-TG) fibroblasts, serum starvation into results a significantly higher percentage calves at term than synchronization early or late For transgenic selected development to post-natal survival weaning This suggests it may necessary coordinate type maximize overall
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