Comparison of early development in utero of cloned fetuses derived from bovine fetal fibroblasts at the G1 and G0/G1 phases
作者： Atsushi Ideta , Koh Hayama , Manami Urakawa , Kanami Tsuchiya , Yoshito Aoyagi
DOI: 10.1016/J.ANIREPROSCI.2010.02.006
关键词: Estrous cycle 、 Blastocyst 、 Gestation 、 Embryo transfer 、 Somatic cell nuclear transfer 、 Biology 、 In utero 、 Gynecology 、 Andrology 、 Embryo 、 Fetus
摘要: Abstract In bovine somatic cell nuclear transfer (NT), embryos are more likely to develop full term when they derived from fibroblasts at the G1 phase instead of cells G0/G1 phase. To better understand reason for this difference, we examined morphological development in early pregnancy NT using (G1-NT embryos) and (G0/G1-NT embryos). Blastocysts G0/G1-NT were transferred recipient heifers, conceptuses day 50 gestation retrieved non-surgically prostaglandin F2α oxytocin. vitro-fertilized (IVF), parthenogenetic artificially inseminated (AI) used as controls. The percentages that developed blastocyst stage did not differ between embryos. Pregnancy rates 30 heifers carrying G1-NT, G0/G1-NT, IVF, AI similar (57–100%). Two returned estrus days gestation, whereas all other pregnancies remained viable. Most fetuses experimental groups (83%) recovered by several PGF2α oxytocin treatments. Recovery normal G1-NT (83%), IVF (80%) (88%) greater than those (33%) (0%). Our results suggest reconstructed with have a high developmental competence time embryo gestation.
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