Factors Affecting the Development of Somatic Cell Nuclear Transfer Embryos in Cattle
作者： Satoshi AKAGI , Kazutsugu MATSUKAWA , Seiya TAKAHASHI
DOI: 10.1262/JRD.2014-057
关键词:
摘要: Nuclear transfer is a complex multistep procedure that includes oocyte maturation, cell cycle synchronization of donor cells, enucleation, fusion, activation and embryo culture. Therefore, many factors are believed to contribute the success development following nuclear transfer. Numerous attempts improve cloning efficiency have been conducted since birth first sheep by somatic However, has remained low, applications limited. In this review, we discuss some affect developmental ability embryos in cattle.
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