Molecular analysis of the trans-activating IE-2 gene of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus

摘要: Abstract A second immediate early (IE) regulatory gene of the baculovirus Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV) has been identified. The IE-2 which is homologous to IE-N Autographa californica MNPV was mapped HindIII fragment OpMNPV between 0.41 1.37 map units. codes for a predicted protein 45,640 Da and analysis amino acid sequence shows that highly basic terminal domain cysteine-rich similar zinc finger motif also found in proteins GC30 PE-38. I E-2 expressed as 1.3-kb transcript detectable by 0.5 hr postinfection (hr p.i.), reached maximum steady state levels 6 p.i., declined slightly 48 p.i. Cis-acting 5′ sequences were analyzed deletion promoter linked reporter chloramphenicol acetyl transferase. Maximum expression obtained when contained 275 upstream from transcriptional start site, trans-Activation revealed trans-activated IE-1 addition appeared be autoregulatory.