miR-675 promotes odontogenic differentiation of human dental pulp cells by epigenetic regulation of DLX3.
作者： Li Zeng , Na Zhao , Fang Li , Dong Han , Yang Liu
DOI: 10.1016/J.YEXCR.2018.03.035
关键词: microRNA 、 Methylation 、 Biology 、 Epigenetics 、 DLX3 、 Real-time polymerase chain reaction 、 Gene knockdown 、 Cell biology 、 Western blot 、 DNMT3B
摘要: Abstract In a previous study, we showed that microRNA-675 (miR-675) was significantly down-regulated in patients with tricho-dento-osseous (TDO) syndrome. One of the main features TDO syndrome is dentin hypoplasia. Thus, hypothesize miR-675 plays role development. this determined odontogenic differentiation human dental pulp cells (hDPCs). Stable overexpression and knockdown hDPCs were performed using recombinant lentiviruses containing U6 promoter-driven short hairpin-miR675 expression cassettes, respectively. Alkaline phosphatase (ALP) assay, Alizarin red staining quantitative polymerase chain reaction (qPCR), Western blot analysis, immunofluorescent revealed promotive effects on hDPCs. Further, found facilitates process by epigenetic regulation distal-less homeobox (DLX3). for first time, regulates inhibiting DNA methyltransferase 3 beta (DNMT3B)-mediated methylation DLX3. Our findings uncover an unanticipated regulatory changes DLX3 provide novel insight into hypoplasia feature patients.
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