Specific association between the human DNA repair proteins XPA and ERCC1

摘要: Abstract Processing of DNA damage by the nucleotide-excision repair pathway in eukaryotic cells is most likely accomplished multiprotein complexes. However, nature these complexes and details molecular interactions between factors are for part unknown. Here, we demonstrate both vivo, using two-hybrid system, vitro, recombinant proteins, that human XPA ERCC1 specifically interact. In addition, report an initial determination domains mediate this interaction. These results suggest may play a role localization or loading incision complex, composed possibly other factors, onto damaged site.