RNA interference against gut osmoregulatory genes in phloem-feeding insects.

摘要: In planta RNAi (i.e. plants engineered to synthesize active molecules) has great potential as a strategy control insect crop pests. This study investigated the impact of against osmoregulatory genes expressed in gut two phloem-feeding species, green peach aphid Myzus persicae and potato/tomato psyllid Bactericera cockerelli. The target comprising candidate sucrase, aquaporin sugar transporter were identified by mining genomic transcriptomic datasets for orthologous empirically-tested pea Acyrthosiphon pisum. Insects feeding on with exhibited elevated hemolymph osmotic pressure (a predicted effect perturbed function) some reduction performance, especially offspring production M. mortality B. cockerelli, associated up 50% mean expression genes. effects particularly pronounced insects treated multiple genes, i.e. combinatorial RNAi, suggesting that partial silencing related roles can yield greater functional impairment than single gene. These results demonstrate but further advances improve efficacy are required achieve effective pest control.