Evolutionary conservation of candidate osmoregulation genes in plant phloem sap‐feeding insects

摘要: The high osmotic pressure generated by sugars in plant phloem sap is reduced phloem-feeding aphids sugar transformations and facilitated water flux the gut. genes mediating these osmoregulatory functions have been identified validated empirically pea aphid Acyrthosiphon pisum: sucrase 1 (SUC1), a glycoside hydrolase family 13 (GH13), aquaporin (AQP1), member of Drosophila integral protein (DRIP) aquaporins. Here, we describe molecular analysis GH13 AQP representatives four groups: (Myzus persicae), coccids (Planococcus citri), psyllids (Diaphorina citri, Bactericera cockerelli) whiteflies (Bemisia tabaci MEAM1 MED). A single candidate GH13-SUC gene DRIP-AQP were genome/transcriptome most insects tested criteria sequence motif expression Exceptionally, psyllid Ba. cockerelli transcriptome included gut-expressed Pyrocoelia rufa (PRIP)-AQP, but has no transcripts, suggesting that PRIP-AQP recruited for function this insect. This study indicates phylogenetically related SUC may generally mediate diverse insects, provides empirical validation development as targets disruption pest species.