PEGylation with the thiosuccinimido butylamine linker significantly increases the stability of haloalkane dehalogenase DhaA
作者： Yuan-Zhong Zhao , Wei-Li Yu , He Zheng , Xuan Guo , Nan Guo
DOI: 10.1016/J.JBIOTEC.2017.05.022
关键词: Ionic strength 、 PEGylation 、 Chemistry 、 Stereochemistry 、 Butylamine 、 Linker 、 Haloalkane dehalogenase 、 Combinatorial chemistry 、 Protonation 、 Methylamine 、 Thermal stability
摘要: Abstract Haloalkane dehalogenase (HLD) can catalyze the hydrolytic dehalogenation of halogenated compounds. However, HLD suffers from poor stability to resist environmental stress. PEGylation is an effective approach enhance enzymes. The linker important stabilization factor PEGylation. Thus, linkers PEGylated were optimized improve in present study. haloalkane DhaAs with methylamine (Ml), carbamate (Cm) and thiosuccinimido butylamine (Tb) prepared, respectively. effects Ml, Cm Tb on investigated under different stresses. Among three linkers, showed highest efficacy DhaA. significantly increased thermal DhaA by slowing its structural unfolding, pH protonation process. In addition, maximum resistance high ionic strength (1 M NaCl) organic solvent (40% DMSO). general interest effectively proteins, particularly protein stability.
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