Detection of carbohydrates by electrospray ionization-ion mobility spectrometry following microbore high-performance liquid chromatography

摘要: Abstract This paper reports the first example of electrospray ion mobility spectrometry as a detection method for HPLC separation, demonstrating its potential quantitative and selective non-volatile non-chromophoric organic compounds. Reduced constants (K0) 21 carbohydrates, including simple sugars, sugar alcohols amino were determined to range from 0.68 1.37 cm2 V−1 s−1. Minimum detectable quantities measured low 5.8·10−14 mol d (+)-cellobiose high 8.2·10−11 l -iditol. A plot carbohydrate mass with respect inverse indicated that, approximation, was inversely proportional most carbohydrates. However, in several cases, isomeric separation could be achieved by spectrometry, that is based on size shape molecule rather than molecular mass. Other notable exceptions mobility-mass relation s- -maltose (+)-cellobiose, which exhibited significantly lower mobilities expected, maltoheptaose, higher expected. Dimerization multiple charges may have caused these deviations mass-mobility correlation. Parametric optimization coupled further basic investigations this ionization should lead novel approach analytical determination