Separation of beta-human chorionic gonadotropin and immunoglobulin G by a miniaturized size exclusion chromatography column
作者： Yongmo Yang , Junseok Chae
DOI: 10.1063/1.3129871
关键词: Resolution (mass spectrometry) 、 Polydimethylsiloxane 、 Size-exclusion chromatography 、 Immunoglobulin G 、 Chromatography 、 Lab-on-a-chip 、 Chemistry 、 Sample preparation 、 Analyte 、 Gonadotropin
摘要: This report describes a miniaturized size exclusion chromatography column that effectively preseparates raw samples for medical point-of-care testing (POCT) devices. The minicolumn is constructed of polydimethylsiloxane fabricated on glass slide. separates 300 ng/ml beta-human chorionic gonadotropin (β-hCG) from an immunoglobulin G (IgG)-rich solution (100 μg/ml) in 7.7 min, with 2.23 resolution and 0.018 mm plate height. complete analyte discrimination shows potential the sample preparation stage POCT devices cancer screening, prognosis, monitoring.
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