Protective effects of ginsenoside Rg1 against hydrogen peroxide-induced injury in human neuroblastoma cells
作者： Li-ping Chen , Zhi-gao Sun , Fa-wei Wang , Cheng-yong Xu , Miao Geng
关键词: Ginseng 、 Hydrogen peroxide 、 Superoxide dismutase 、 Lactate dehydrogenase 、 Hsp70 、 Mechanism of action 、 Pharmacology 、 Caspase 3 、 Apoptosis 、 Biochemistry 、 Medicine
摘要: The active ingredient of ginseng, ginsenosides Rg1, has been shown to scavenge free radicals and improve antioxidant capacity. This study hypothesized that Rg1 a protective role in human neuroblastoma cells injured by H2O2. Ginsenosides at different concentrations (50 100 μM) was used treat H2O2 (150 μM)-injured SH-SY5Y cells. Results demonstrated ginsenoside elevated the survival rate H2O2, diminished amount leaked lactate dehydrogenase, increased superoxide dismutase activity. Ginsenoside effectively suppressed caspase-3 immunoreactivity, contributed heat shock protein 70 gene expression, dose-dependent manner. These results indicate effects on its mechanism action is associated with anti-oxidation inhibition apoptosis.
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