Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application

摘要: In this study, we extracted and purified components in the Angong Niuhuang pill. Then applied transdermal enhancers to stickers by modern technology. The sticker includes extracts from curcuma, berberine hydrochloride, baicalin, geniposide, borneol, musk. at different point application doses (1.35, 2.7, 5.4 g/kg) were administered Dazhui (DU14), Qihai (RN6) Mingmen (DU4). Rats dose acupuncture groups continuously for 7 days. a middle cerebral artery occlusion model was prepared simulating human ischemia. Twelve hours later, expressions of Bcl-2, Bax p53 protein hippocampal CA1 detected with immunohistochemistry. expression level an anti-apoptotic protein, significantly increased high-, medium- low-dose group, while pro-apoptotic proteins, p53, decreased compared rats; Bcl-2/Bax ratio increased. difference noticeable high-dose which showed statistical group group. Our experimental findings indicate that promotes inhibits area rats after focal Thus, Niuhang protects brain tissues