The anti-human immunodeficiency virus agent 3'-fluorothymidine induces DNA damage and apoptosis in human lymphoblastoid cells.
作者： R Sundseth , S S Joyner , J T Moore , R E Dornsife , I K Dev
DOI: 10.1128/AAC.40.2.331
关键词: Fragmentation (cell biology) 、 Biology 、 Agarose gel electrophoresis 、 Cell culture 、 Virology 、 DNA damage 、 Thymidine 、 Molecular biology 、 Programmed cell death 、 Apoptosis 、 DNA fragmentation
摘要: Patients infected with the human immunodeficiency virus experienced severe hematopoietic toxicity after treatment deoxynucleoside analog 3'-fluorothymidine (FLT). Using several methods for analysis of genome integrity, including histochemical staining 3' ends DNA and both conventional pulsed-field agarose gel electrophoresis, we demonstrated that FLT caused extensive fragmentation in CEM cells was not observed when these were treated other, less toxic thymidine analogs. In addition, a distinctive pattern small fragments is characteristic process programmed cell death genomic FLT. We conclude induces apoptosis line origin, offer this observation as possible explanation vivo.
sci-hub.se 参考文章(23)
F W Perrino, H Miller, K A Ealey, Identification of a 3'-->5'-exonuclease that removes cytosine arabinoside monophosphate from 3' termini of DNA. Journal of Biological Chemistry. ,vol. 269, pp. 16357- 16363 ,(1994) , 10.1016/S0021-9258(17)34015-2
C H Chen, Y C Cheng, Delayed cytotoxicity and selective loss of mitochondrial DNA in cells treated with the anti-human immunodeficiency virus compound 2',3'-dideoxycytidine. Journal of Biological Chemistry. ,vol. 264, pp. 11934- 11937 ,(1989) , 10.1016/S0021-9258(18)80156-9
Lang Je, Barrett Jc, Maronpot Rr, Preston Ga, Regulation of Apoptosis by Low Serum in Cells of Different Stages of Neoplastic Progression: Enhanced Susceptibility after Loss of a Senescence Gene and Decreased Susceptibility after Loss of a Tumor Suppressor Gene Cancer Research. ,vol. 54, pp. 4214- 4223 ,(1994)
Chin-Ho Chen, Yung-Chi Cheng, M. Vazquez-Padua, Effect of anti-human immunodeficiency virus nucleoside analogs on mitochondrial DNA and its implication for delayed toxicity. Molecular Pharmacology. ,vol. 39, pp. 625- 628 ,(1991)
R. G. Morris, A. H. Wyllie, M. J. Arends, Apoptosis. The role of the endonuclease American Journal of Pathology. ,vol. 136, pp. 593- 608 ,(1990)
W GORCZYCA, S BRUNO, RJ DARZYNKIEWICZ, JP GONG, Z DARZYNKIEWICZ, DNA strand breaks occurring during apoptosis - their early insitu detection by the terminal deoxynucleotidyl transferase and nick translation assays and prevention by serine protease inhibitors. International Journal of Oncology. ,vol. 1, pp. 639- 648 ,(1992) , 10.3892/IJO.1.6.639
John F. R. Kerr, Clay M. Winterford, Brian V. Harmon, Apoptosis. Its significance in cancer and cancer Therapy Cancer. ,vol. 73, pp. 2013- 2026 ,(1994) , 10.1002/1097-0142(19940415)73:8<2013::AID-CNCR2820730802>3.0.CO;2-J
C. E. Canman, H. Y. Tang, D. P. Normolle, T. S. Lawrence, J. Maybaum, Variations in patterns of DNA damage induced in human colorectal tumor cells by 5-fluorodeoxyuridine: implications for mechanisms of resistance and cytotoxicity Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 10474- 10478 ,(1992) , 10.1073/PNAS.89.21.10474
Peng Huang, William Plunkett, Fludarabine- and gemcitabine-induced apoptosis: incorporation of analogs into DNA is a critical event. Cancer Chemotherapy and Pharmacology. ,vol. 36, pp. 181- 188 ,(1995) , 10.1007/BF00685844
Anna KARLSSON, Peter REICHARD, Fritz ECKSTEIN, Hydroxyurea increases the phosphorylation of 3′‐fluorothymidine and 3′‐azidothymidine in CEM cells FEBS Journal. ,vol. 186, pp. 689- 694 ,(1989) , 10.1111/J.1432-1033.1989.TB15261.X