MOLECULAR IDENTIFICATION OF AEROMONAS SPP ISOLATED FROM PATIENTS WITH DIARRHEA AT SANTA MARIA-RS, BRAZIL

作者: Cyntia Maria Telles Fadel-picheth , Karoline De Campos Prediger , Cibelle De Borba Dallagassa , Roberto Christ Vianna Santos , Adriana Medianeira Rossato

DOI:

关键词: MicrobiologyDiarrheaAeromonasAeromonas speciesMolecular identificationBiovarBiology

摘要: Objective: The aim of the study was to determine frequency Aeromonas spp. in stool samples outpatients with gastroenteritis attended by clinical laboratories at Santa Maria-RS, Brazil. Methods: In order evaluate this frequency, 767 were processed conventional methods as preconized, and suspected strains submitted molecular characterization 16SrRNA PCR-RFLP method. Results: isolated from 14 (1.8%) cultures identified A. caviae (04), hydrophila (03), veronii biovar sobria (01) Six presented atypical patterns, therefore Conclusion: is part bacteria associated diarrhea results indicates that least 3 species are involved disease.

参考文章(14)
Maria Elena Martino, Luca Fasolato, Filomena Montemurro, Enrico Novelli, Barbara Cardazzo, Aeromonas spp.: ubiquitous or specialized bugs? Environmental Microbiology. ,vol. 16, pp. 1005- 1018 ,(2014) , 10.1111/1462-2920.12215
Antonio J. Martínez-Murcia, Nuria Borrell, María-José Figueras, Typing of clinical and environmental Aeromonas veronii strains based on the 16S–23S rDNA spacers Fems Immunology and Medical Microbiology. ,vol. 28, pp. 225- 232 ,(2000) , 10.1111/J.1574-695X.2000.TB01481.X
Monica Surek, Bruno S Vizzotto, Emanuel M Souza, Fábio de O Pedrosa, Cibelle B Dallagassa, Sonia MSS Farah, Cyntia MT Fadel-Picheth, None, Identification and antimicrobial susceptibility of Aeromonas spp. isolated from stool samples of Brazilian subjects with diarrhoea and healthy controls. Journal of Medical Microbiology. ,vol. 59, pp. 373- 374 ,(2010) , 10.1099/JMM.0.014258-0
Ivani MF Guerra, Raquel Fadanelli, Manuela Figueiró, Fernando Schreiner, Ana Paula L Delamare, Claudia Wollheim, Sérgio Olavo P Costa, Sergio Echeverrigaray, None, Aeromonas associated diarrhoeal disease in south Brazil: prevalence, virulence factors and antimicrobial resistance Brazilian Journal of Microbiology. ,vol. 38, pp. 638- 643 ,(2007) , 10.1590/S1517-83822007000400011
Jennifer L. Parker, Jonathan G. Shaw, Aeromonas spp. clinical microbiology and disease Journal of Infection. ,vol. 62, pp. 109- 118 ,(2011) , 10.1016/J.JINF.2010.12.003
Frédéric Roger, Brigitte Lamy, Estelle Jumas-Bilak, Angeli Kodjo, Hélène Marchandin, , Ribosomal Multi-Operon Diversity: An Original Perspective on the Genus Aeromonas PLoS ONE. ,vol. 7, pp. e46268- ,(2012) , 10.1371/JOURNAL.PONE.0046268
M J Figueras, L Soler, M R Chacón, J Guarro, A J Martínez-Murcia, Extended method for discrimination of Aeromonas spp. by 16S rDNA RFLP analysis. International Journal of Systematic and Evolutionary Microbiology. ,vol. 50, pp. 2069- 2073 ,(2000) , 10.1099/00207713-50-6-2069
S. L. Abbott, W. K. W. Cheung, J. M. Janda, The Genus Aeromonas: Biochemical Characteristics, Atypical Reactions, and Phenotypic Identification Schemes Journal of Clinical Microbiology. ,vol. 41, pp. 2348- 2357 ,(2003) , 10.1128/JCM.41.6.2348-2357.2003
N Borrell, S G Acinas, M J Figueras, A J Martínez-Murcia, Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16S rRNA genes. Journal of Clinical Microbiology. ,vol. 35, pp. 1671- 1674 ,(1997) , 10.1128/JCM.35.7.1671-1674.1997
Alessia Morandi, Olga Zhaxybayeva, J. Peter Gogarten, Joerg Graf, Evolutionary and Diagnostic Implications of Intragenomic Heterogeneity in the 16S rRNA Gene in Aeromonas Strains Journal of Bacteriology. ,vol. 187, pp. 6561- 6564 ,(2005) , 10.1128/JB.187.18.6561-6564.2005