Isoflavones as Apoptosis Inducers in Human Hepatoma HuH‐7 Cells
作者： Tayyab A Mansoor , Rita M Ramalho , Xuan Luo , Cátia Ramalhete , Cecília MP Rodrigues
DOI: 10.1002/PTR.3498
关键词: Flavanone 、 Biochanin A 、 Biology 、 Prunetin 、 Irilone 、 Formononetin 、 Isoflavones 、 Genistein 、 Biochemistry 、 Programmed cell death
摘要: Nine flavonoids isolated from the ethyl acetate extract of Pycnanthus angolensis were assayed for their potential apoptosis induction activities in human hepatoma HuH-7 cells. These include eight isoflavones, namely irilone (1), tectorigenine (2), formononetin (3), genistein (4), 2′-hydroxybiochanin A (5), mixture biochanin (6) and prunetin (7), 4′,7-dihydroxy-2′-methoxyisoflavan (8), flavanone liguiritigentin (9). Their chemical structures characterized by spectroscopic methods including 2D NMR experiments. Methodology cell death detection included LDH assay, Hoechst staining, TUNEL staining general caspase-3-like activity assay. The compounds tested showed higher profiles cells compared with control. Caspase assays confirmed inducing these flavonoids. Copyright © 2011 John Wiley & Sons, Ltd.
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