Concentration-dependent effects of the soy phytoestrogen genistein on the proteome of cultured cardiomyocytes.
作者： Zeyu Sun , Laurie M. Biela , Karyn L. Hamilton , Kenneth F. Reardon
DOI: 10.1016/J.JPROT.2012.04.001
关键词:
摘要: Abstract The soy-derived phytoestrogen genistein (GEN) has received attention for its potential benefits on the cardiovascular system by providing direct protection to cardiomyocytes against pathophysiological stresses. Here, we employed a proteomic approach study concentration-dependent effects of GEN treatments cardiomyocytes. Cultured HL-1 were treated with low (1 μM) and high (50 μM) concentrations GEN. Proteins pre-fractionated sequential hydrophilic/hydrophobic extraction both protein fractions from each treatment group separated 2D gel electrophoresis (2DE). Overall, approximately 2,700 spots visualized gels. Thirty-nine 99 changed in volume relative controls (p
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