Extracellular-regulated kinase 1/2, Jun N-terminal kinase, and c-Jun are involved in NF-kappa B-dependent IL-6 expression in human monocytes.

摘要: In the present study we investigated possible involvement of mitogen-activated protein kinase family members extracellular-regulated 1/2 (ERK1/2) and c-Jun N-terminal (JNK) in mediating IL-6 gene expression human monocytes, particular their role enhancing NF-κB activity. Freshly isolated monocytes treated with phosphatase inhibitor okadaic acid secreted high levels protein, which coincided enhanced binding activity as well phosphorylation activation ERK1/2 JNK proteins. The ERK pathway-specific PD98059 inhibited secretion from monocytes. Transient overexpression inactive mutants either Raf-1 or JNK1 showed that both pathways were involved κB-dependent promoter By using PD98059, demonstrated Raf1/MEK1/ERK1/2 pathway did not affect DNA but, rather, acted at level transcriptional NF-κB. Interestingly, it was shown NF-κB-mediated transcription, context on its own, dependent serine interaction protein. We conclude acid-induced is least partly mediated through pathway-dependent capacity. Our results suggest may regulate transcription c-Jun.