The effects of different sample labelling methods on signal intensities of a 60-mer diagnostic microarray
作者： Xiao-Yang Mo , Wen-Li Ma , Ling Li , Qiu-Lin Xu , Ya-Li Zhang
DOI: 10.1016/J.JVIROMET.2005.11.017
关键词: Primer (molecular biology) 、 Complementary DNA 、 Labelling 、 Molecular biology 、 Diagnostic microarray 、 Signal 、 Oligonucleotide 、 Human influenza 、 Biology
摘要: Abstract The effects of four different labelling methods on signal intensities a 60-mer diagnostic microarray were studied. Eighty virus-specific oligonucleotide probes for human influenza virus prepared in an array 15 × 16 spots. RNA samples from cultured strains labelled with methods, including direct cDNA (DL), universal primer (UPL), restriction display (DL–RD), and Cy-dUTP incorporated (IL–RD) color format. background-subtracted five replicate hybridization experiments each method analyzed using one-way analysis variance (one-way ANOVA) linear regression techniques. effect sample was significant ( p
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