Influence of dilution rate and induction of cloned gene expression in continuous fermentations of recombinant yeast
作者： Nancy Anderson Da Silva , James E. Bailey
关键词: Gene expression 、 Gene product 、 Biochemistry 、 Steady state (chemistry) 、 Saccharomyces cerevisiae 、 Plasmid 、 Inducer 、 Biology 、 Yeast 、 Dilution 、 Biotechnology 、 Applied Microbiology and Biotechnology 、 Bioengineering
摘要: The effects of growth rate on cloned gene product synthesis in recombinant Saccharomyces cerevisiae have been studied continuous culture. plasmid employed contains a yeast GAL10-CYC1 hybrid promoter directing expression the E. coli lacZ gene. beta-Galactosidase production was therefore controlled by galactose regulatory circuit, and induction process its were at various dilution rates. At all rates stability decreased with expression. In some instances, two induced "steady states" observed, first 10-15 residence times after second 40-50 times. steady state characterized greater biomass concentration lower beta-galactosidase specific activity relative to "steady-state." increased as reduced, despite flow stability, overall productivity (activity/L/hr) substantially higher low rate. Important factors influencing trends glucose (inducer) concentrations vessel inducer metabolism.
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