Primary structure of the low-molecular-weight carbohydrate chains of Helix pomatia alpha-hemocyanin. Xylose as a constituent of N-linked oligosaccharides in an animal glycoprotein.
作者： J A van Kuik , H van Halbeek , J P Kamerling , J F Vliegenthart
DOI: 10.1016/S0021-9258(17)38672-6
关键词: Carbohydrate 、 Galactose 、 Xylose 、 Stereochemistry 、 Biochemistry 、 Mannose 、 Glycan 、 Glycoprotein 、 Fucose 、 Helix pomatia 、 Chemistry 、 Cell biology 、 Molecular biology
摘要: alpha-Hemocyanin of Helix pomatia is a copper-containing glycoprotein which serves as an oxygen carrier in the hemolymph. Its carbohydrate moiety has constituents fucose, xylose, 3-O-methylgalactose, mannose, galactose, N-acetylgalactosamine, and N-acetyl-glucosamine residues. Alkaline borhydride did not split off any material, suggesting absence O-glycosidic chains. The N-glycosidic chains this were liberated by hydrazinolysis Pronase digest then fractionated alditols on Bio-Gel P-4. fractions containing low-molecular-weight glycans investigated 500-MHz 1H NMR spectroscopy conjunction with sugar methylation analysis. largest, most abundant, compound was established to be: (Formula: see text). Another characterized afuco analogue structure. H. alpha-hemocyanin first example animal having xylose constituent
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