Integration of Metabolomics and Subcellular Organelle Expression Microarray to Increase Understanding the Organic Acid Changes in Post-harvest Citrus Fruit

摘要: Citric acid plays an important role in fresh fruit flavor and its adaptability to post-harvest storage conditions. In order explore organic regulatory mechanisms citrus fruit, systematic biological analyses were conducted on stored Hirado Buntan Pummelo (HBP; Citrus grandis) fruits. High-performance capillary electrophoresis, subcellular organelle expression microarray, real-time quantitative reverse transcription polymerase chain reaction, gas chromatography mass spectrometry (GC-MS), conventional physiological biochemical undertaken. The results showed that the concentration of acids HBP underwent a regular fluctuation. GC-MS-based metabolic profiling indicated succinic acid, γ-aminobutyric (GABA), glutamine contents increased, but 2-oxoglutaric content declined, which further confirmed GABA shunt may have some roles catabolism processes. addition, was significantly correlated with senescence-related processes, such as hydrogen peroxide well superoxide dismutase peroxidase activities, could be regarded parameters for measuring senescence