Identification and Differential Abundance of Mitochondrial Genome Encoding Small RNAs (mitosRNA) in Breast Muscles of Modern Broilers and Unselected Chicken Breed.

摘要: Background: Although small non-coding RNAs are mostly encoded by the nuclear genome, thousands of mitochondrial termed as mitosRNAs were recently reported in human, mouse and trout. In this study, we first identified chicken breast muscle using RNA sequencing method differential abundance was analyzed between modern pedigree male (PeM) broilers (characterized rapid growth large mass) foundational Barred Plymouth Rock (BPR) chickens slow mass). Methods: Small performed with total extracted from muscles PeM BPR (n = 6 per group) 1 × 50 bp single end read Illumina sequencing. Raw reads processed quality assessment, adapter trimming, alignment to genome (GenBank Accession: X52392.1) NGen program. Further statistical analyses JMP Genomics 8. Differentially expressed (DE) confirmed quantitative PCR. Results: Totals 183,416 unique sequences potential mitosRNAs. After stringent filtering processes, 117 showing >100 raw counts abundantly produced all 37 genes (except D-loop region) length ranged 22 46 nucleotides. Of those, 44 significantly altered compared those BPR: higher except 16S-rRNA gene. Possibly, may be due a content BPR. Our data demonstrate that addition known genes, also encodes abundant mitosRNAs, play an important regulatory role via gene expression control.