Proteomics of Breast Muscle Tissue Associated with the Phenotypic Expression of Feed Efficiency within a Pedigree Male Broiler Line: I. Highlight on Mitochondria

摘要: As feed represents 60 to 70% of the cost raising an animal market weight, efficiency (the amount dry weight intake wet gain) remains important genetic trait in agriculture. To gain greater understanding cellular mechanisms (FE), shotgun proteomics was conducted using in-gel trypsin digestion and tandem mass spectrometry on breast muscle samples obtained from pedigree male (PedM) broilers exhibiting high (FE) or low FE phenotypes (n = 4 per group). The group had body (P 0.004) but consumed same 0.30) 6 7 wk resulting higher < 0.001). Over 1800 proteins were identified, which 152 different 0.05) by at least 1.3 fold ≤ 15 between phenotypes. Data analyzed for a modified differential expression (DE) metric (Phenotypic Impact Factors PIF) interpretation protein data facilitated Ingenuity Pathway Analysis (IPA) program. In entire set, 228 mitochondrial identified whose collective indicates phenotype (binomial probability P 0.00001). Within top up down 5% PIF molecules dataset, there mitoproteome up-regulated only 5 down-regulated phenotype. enrichment analysis also dysfunction oxidative phosphorylation as number 1 differentially expressed canonical pathways (up-regulated FE) proteomic dataset. Upstream (based DE downstream molecules) predicted that insulin receptor, like growth receptor 1, nuclear factor, erythroid 2-like 2, AMP activated kinase (α subunit), progesterone triiodothyronine would be whereas rapamycin independent companion target rapamycin, mitogen 4, serum response factor inhibited results provide additional insight into fundamental molecular landscape well further support role mitochondria phenotypic FE. Funding provided USDA-NIFA (#2013–01953), Arkansas Biosciences Institute (Little Rock, AR), McMaster Fellowship (AUS WB) Agricultural Experiment Station (Univ. Arkansas, Fayetteville).