Single-filament kinetic studies provide novel insights into regulation of actin-based motility

摘要: Polarized assembly of actin filaments forms the basis actin-based motility and is regulated both spatially temporally. Cells use a variety mechanisms by which intrinsically slower processes are accelerated, faster ones decelerated, to match rates observed in vivo. Here we discuss how kinetic studies individual reactions cycles that drive remodeling have provided mechanistic quantitative understanding such processes. We specifically consider key barbed-end regulators as capping protein formins illustrative examples. compare contrast different approaches, traditional pyrene-polymerization bulk assays, well more recently developed single-filament single-molecule imaging approaches. Recent development novel biophysical methods for sensing applying forces will future allow us address very important relationship between mechanical stimulus kinetics motility.