The interaction between Trypanosoma rangeli and the nitrophorins in the salivary glands of the triatomine Rhodnius prolixus (Hemiptera; Reduviidae)

摘要: Abstract The parasite Trypanosoma rangeli develops in the intestinal tract of triatomines and, particularly species genus Rhodnius , invades hemolymph and salivary glands, where subsequent metacyclogenesis takes place. Many aspects interaction between T. rangeli are still unclear, especially concerning development glands how interacts with saliva. In this work, we describe new findings on process infection impact saliva composition. To ensure a complete (intestinal tract, glands), 3rd instar prolixus nymphs were fed blood containing epimastigotes using an artificial feeder. After molt to 4th instar, inoculated hemolymph. results showed that flagellates started invade by 7th day after injection. percentage trypomastigotes inside continuously increased until 25th day, at which time more than 95% forms present. contents from -infected insects pH was significantly acidic ( . addition, four major nitrophorins (NPs 1–4) knocked down RNAi their suppression impacted survival point burden R. prolixus reduced 3-fold. These indicated these parasites most likely non-specifically incorporated proteins present as nutrients, without impairing biosynthesis antihemostatic molecules.