Penetration of the salivary glands of Rhodnius domesticus Neiva & Pinto, 1923 (Hemiptera: Reduviidae) by Trypanosoma rangeli Tejera, 1920 (Protozoa: Kinetoplastida).
作者： Rosane M. S. Meirelles , Andrea Henriques-Pons , Maurilio J. Soares , Mário Steindel
DOI: 10.1007/S00436-005-1433-4
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摘要: Penetration of the heteroxenous protozoan Trypanosoma rangeli into salivary glands its invertebrate host Rhodnius domesticus has been investigated here using different approaches. Electron microscopy showed that epimastigotes coming from insect hemocoel cross basal lamina surrounds and penetrate through gland cells cytoplasm. After reaching lumen, epimastigote forms remain adhered to cell microvilli by their flagella, while metacyclic trypomastigotes are found swimming free in saliva. Analysis flow cytometry, western blotting hemolytic activity allowed demonstrate presence T. a molecule with antigenic cross-reactivity murine perforin, which could be used parasites reach lumen. This molecule, we named as rangelysin, 120 kDa molecular weight, is able induce hemolysis only acidic pH, produced both trypomastigote forms.
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sci-hub.se 参考文章(47)
A Ludwig, Cytolytic toxins from gram-negative bacteria. Microbiología (Madrid, Spain). ,vol. 12, pp. 281- 296 ,(1996)
Leonardo Koerich, Priscilla Emmanuelle-Machado, Kelly Santos, Edmundo C Grisard, Mário Steindel, None, Differentiation of Trypanosoma rangeli: high production of infective Trypomastigote forms in vitro. Parasitology Research. ,vol. 88, pp. 21- 25 ,(2002) , 10.1007/S004360100501
Vern B Carruthers, Armed and dangerous: Toxoplasma gondii uses an arsenal of secretory proteins to infect host cells. Parasitology International. ,vol. 48, pp. 1- 10 ,(1999) , 10.1016/S1383-5769(98)00042-7
E.C. Lynch, I.M. Rosenberg, C. Gitler, An ion-channel forming protein produced by Entamoeba histolytica. The EMBO Journal. ,vol. 1, pp. 801- 804 ,(1982) , 10.1002/J.1460-2075.1982.TB01250.X
Rosa Nickel, Claudia Ott, Thomas Dandekar, Matthias Leippe, Pore-forming peptides of Entamoeba dispar. Similarity and divergence to amoebapores in structure, expression and activity. FEBS Journal. ,vol. 265, pp. 1002- 1007 ,(2001) , 10.1046/J.1432-1327.1999.00807.X
James A. Roth, Virulence mechanisms of bacterial pathogens. Virulence mechanisms of bacterial pathogens.. ,(1988)
J D Young, T M Young, L P Lu, J C Unkeless, Z A Cohn, Characterization of a membrane pore-forming protein from Entamoeba histolytica. Journal of Experimental Medicine. ,vol. 156, pp. 1677- 1690 ,(1982) , 10.1084/JEM.156.6.1677
Felipe Guhl, Carlos Jaramillo, Julio César Carranza, Gustavo A Vallejo, None, Molecular characterization and diagnosis of trypanosoma cruzi and T. rangeli. Archives of Medical Research. ,vol. 33, pp. 362- 370 ,(2002) , 10.1016/S0188-4409(02)00380-6
Shibo Jiang, David M. Ojcius, J.D.-E. Young, Perforin binding to cells and lipid membranes determined by a simple competition assay Journal of Immunological Methods. ,vol. 126, pp. 29- 37 ,(1990) , 10.1016/0022-1759(90)90008-J
Pier Luigi Fiori, Paola Rappelli, Maria Filippa Addis, Antonio Sechi, Piero Cappuccinelli, Trichomonas vaginalis haemolysis: pH regulates a contact-independent mechanism based on pore-forming proteins. Microbial Pathogenesis. ,vol. 20, pp. 109- 118 ,(1996) , 10.1006/MPAT.1996.0010