Supramolecular Control of Split-GFP Reassembly by Conjugation of β-Cyclodextrin and Coumarin Units
作者： Seiji Sakamoto , Kazuaki Kudo
DOI: 10.1021/JA802313A
关键词: Beta-Cyclodextrins 、 Protein folding 、 Stereochemistry 、 Supramolecular chemistry 、 Chemistry 、 Green fluorescent protein 、 Ligand 、 Circular dichroism 、 Peptide 、 Protein engineering
摘要: The design of proteins whose structure and function can be manipulated by binding with specific ligands has been great interest in the field protein engineering. Some successful examples small-molecule-dependent have reported, but their ligand-binding domains mainly limited to those derived from natural proteins. introduction synthetic components for ligand responsiveness may expand versatility In this study, we designed constructed a fragmented green fluorescent (split GFP) reassembly could modulated non-natural supramolecular interaction. split GFP, β-cyclodextrin (βCDx) coumarin units were introduced into C-terminal fragment (residues 214−230) GFP. peptide βCDx coumarin, DC-M2-βCDx, which contains both host guest moieties same chain, formed an intramolecular inclusion complex absence exogenous molecules. ...
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