Investigation of the role of alginate containing high guluronic acid on osteogenic differentiation capacity of human umbilical cord Wharton's jelly mesenchymal stem cells.
作者： Darioush Bijan Nejad , Saeed Azandeh , Rezvan Habibi , Esrafil Mansouri , Vahid Bayati
DOI: 10.1080/02652048.2017.1393115
关键词: Alkaline phosphatase 、 Viability assay 、 Cell encapsulation 、 MTT assay 、 Chemistry 、 Staining 、 Mesenchymal stem cell 、 Wharton's jelly 、 ALIZARIN RED 、 Andrology
摘要: AbstractBackground and aim: Cell encapsulation using biodegradable material has promising results for tissue engineering. Since pressure is an effective factor on stem cell behaviour various concentrations of alginate create different pressures the cells, therefore our goal was to evaluate mechanical effect 1/2% (w/v) 1/8% containing high guluronic acid viability osteogenic capacity HUCWJ cells.Methods: evaluated by MTT assay after 1, 7 14 days. Alkaline phosphatase activity alkaline kit 21 Alizarin red S staining performed calcium deposition among histological section.Results: showed significant difference in mean rates between groups day (p < 0.05). higher group 1.8%. In addition, there statistically ALP 1.8% compared 1.2%.
sci-hub.se 参考文章(39)
Marita Westhrin, Encapsulation of Human Mesenchymal Stem Cells in Phosphate Mineralized Alginate Beads 79. ,(2011)
Batool Hashemibeni, Farzaneh Sadeghi, Vahid Goharian, Ebrahim Esfandiari, Mohammad Mardani, Shiva Roshankhah, Fariba Heidary, An animal model study for bone repair with encapsulated differentiated osteoblasts from adipose-derived stem cells in alginate. Iranian Journal of Basic Medical Sciences. ,vol. 17, pp. 854- 859 ,(2014) , 10.22038/IJBMS.2014.3718
Jenna L. Wilson, Mohamad Ali Najia, Rabbia Saeed, Todd C. McDevitt, Alginate encapsulation parameters influence the differentiation of microencapsulated embryonic stem cell aggregates Biotechnology and Bioengineering. ,vol. 111, pp. 618- 631 ,(2014) , 10.1002/BIT.25121
Dong-Hwan Kim, Dae-Duk Kim, In-Soo Yoon, Proliferation and chondrogenic differentiation of human adipose-derived mesenchymal stem cells in sodium alginate beads with or without hyaluronic acid Journal of Pharmaceutical Investigation. ,vol. 43, pp. 145- 151 ,(2013) , 10.1007/S40005-013-0059-2
Liang Zhao, Michael D. Weir, Hockin H.K. Xu, Human umbilical cord stem cell encapsulation in calcium phosphate scaffolds for bone engineering. Biomaterials. ,vol. 31, pp. 3848- 3857 ,(2010) , 10.1016/J.BIOMATERIALS.2010.01.093
Dolores Baksh, Raphael Yao, Rocky S. Tuan, Comparison of Proliferative and Multilineage Differentiation Potential of Human Mesenchymal Stem Cells Derived from Umbilical Cord and Bone Marrow Stem Cells. ,vol. 25, pp. 1384- 1392 ,(2007) , 10.1634/STEMCELLS.2006-0709
Jinchen Sun, Huaping Tan, None, Alginate-Based Biomaterials for Regenerative Medicine Applications. Materials. ,vol. 6, pp. 1285- 1309 ,(2013) , 10.3390/MA6041285
Saeed Azandeh, Mahmoud Orazizadeh, Mahmoud Hashemitabar, Ali Khodadadi, Ali Akbar Shayesteh, Darioush Bijan Nejad, Anneh Mohammad Gharravi, Elham Allahbakhshi, Mixed enzymatic-explant protocol for isolation of mesenchymal stem cells from Wharton’s jelly and encapsulation in 3D culture system Journal of Biomedical Science and Engineering. ,vol. 2012, pp. 580- 586 ,(2012) , 10.4236/JBISE.2012.510071
Hwai-Shi Wang, Shih-Chieh Hung, Shu-Tine Peng, Chun-Chieh Huang, Hung-Mu Wei, Yi-Jhih Guo, Yu-Show Fu, Mei-Chun Lai, Chin-Chang Chen, Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical Cord Stem Cells. ,vol. 22, pp. 1330- 1337 ,(2004) , 10.1634/STEMCELLS.2004-0013
MATTHEUS F. A. GOOSEN, Physico-chemical and mass transfer considerations in microencapsulation. Annals of the New York Academy of Sciences. ,vol. 875, pp. 84- 104 ,(1999) , 10.1111/J.1749-6632.1999.TB08496.X