Proliferation and chondrogenic differentiation of human adipose-derived mesenchymal stem cells in sodium alginate beads with or without hyaluronic acid
作者： Dong-Hwan Kim , Dae-Duk Kim , In-Soo Yoon
DOI: 10.1007/S40005-013-0059-2
关键词:
摘要: Human adipose-derived mesenchymal stem cells (AD-MSCs) have attracted much interest as an alternative to autologous chondrocytes and bone marrow-derived for cell-based therapy repair cartilage defects. Sodium alginate (SA) beads been widely known a conventional cell delivery system repair. Hyaluronic acid (HA) has induce proliferation chondrogenic differentiation. Herein, we prepared AD-MSCs-encapsulating SA with HA (SA–HA beads) without (SA beads). Then, the morphology, proliferation, differentiation of AD-MSCs cultured in SA–HA or media were evaluated. There was no discernible difference morphology between beads. However, (MTT optical density DNA contents) (s-GAG contents type II collagen staining) significantly enhanced compared The present results suggest that can be added beads-based systems order improve their chondrogenesis-inducing capacity
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