Novel porous matrix of hyaluronic acid for the three-dimensional culture of chondrocytes

摘要: A novel three-dimensional (3D) scaffold of chemically unmodified hyaluronic acid (HA) with minimum cross-linkage was developed for the culture chondrocytes, thereby to promote cartilage repair. The porous structure observed by scanning electron microscopy (SEM), and pore size controlled fabrication conditions including swelling time composition HA matrix. Rabbit primary chondrocytes human chondrocytic cell lines (C-20/A4) were cultured in matrix investigate whether they can be applied construct tissue vitro. retained spherical morphology this Moreover, results from MTT assay showed good cellular viability within matrix; optical density increased up 28 days, demonstrating that cells continued proliferate inside Phenotypic analysis (RT-PCR, Alcian blue staining quantification s-GAG) when three-dimensionally matrix, expressed transcripts encoding collagen type II aggrecan, produced sulfated glycosaminoglycans (s-GAG), indicating chondrogenic differentiation. new therefore appears as a potentially promising engineering.