Microbiome sequencing: challenges and opportunities for molecular medicine

摘要: ABSTRACTIntroduction: Since the discovery of Polymerase Chain Reaction and pioneering work on 16S ribosomal RNA genes in 1980’s, use molecular techniques to study microbial ecosystems pathogenic microorganisms has been increasing exponentially. Because communities inhabiting various body sites such as skin or intestine can influence our physiology, there a massive interest studying their diversity, functions, role development acute chronic diseases.Area covered: In present review, we gather knowledge sequencing approaches communities, especially human microbiota, give opinions potential limitations, particularly with respect clinical applications.Expert commentary: High-throughput delivered unprecedented views complex but popularization is accompanied by substantial technical hurdles that will need be overcome for efficient implementation routin...