Atomic Force Microscopy of Biological Membranes
作者： Patrick L.T.M. Frederix , Patrick D. Bosshart , Andreas Engel
DOI: 10.1016/J.BPJ.2008.09.046
关键词: Cell membrane 、 Biological membrane 、 Cantilever 、 Adsorption 、 Molecular machine 、 Microelectrode 、 Nanotechnology 、 Membrane 、 Chemistry 、 Microscopy
摘要: Atomic force microscopy (AFM) is an ideal method to study the surface topography of biological membranes. It allows membranes that are adsorbed flat solid supports be raster-scanned in physiological solutions with atomically sharp tip. Therefore, AFM capable observing molecular machines at work. In addition, tip can tethered end a single membrane protein, and forces acting on upon its retraction indicate barriers occur during process protein unfolding. Here we discuss fundamental limitations determined by properties cantilevers, present aspects sample preparation, review results achieved reconstituted native
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