Use of isolated, perfused liver in studies of receptor-mediated endocytosis.
作者： William A. Dunn , Doris A. Wall , Ann L. Hubbard
DOI: 10.1016/0076-6879(83)98151-X
关键词: Endocytosis 、 In vivo 、 Endocytic cycle 、 Ligand (biochemistry) 、 Glycoprotein 、 Biology 、 Asialoglycoprotein 、 Biochemistry 、 Galactose 、 Receptor-mediated endocytosis
摘要: Publisher Summary This chapter describes the use of isolated, perfused liver in studies receptor-mediated endocytosis. The focuses on asialoglycoprotein (ASGP) recognition system rat liver, which mediates clearance glycoproteins having galactose- or N -acetylgalac- tosamine-terminating carbohydrate side chains. dynamics ligand pathway vivo , isolated hepatocytes, and is studied. has been used a variety forms by many investigators for study number functions. perfusion comprises three primary components: (1) temperature-regulation system; (2) pump; (3) gas-permeable fibers mounted beaker assembly. also illustrates step-by-step procedure isolating procedures performing temperature-shift experiments, presents an outline method to label selectively contents endocytic compartments, using as model. selected functions that relate endocytosis are examined.
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