Ultra-high-field (9.4 T) MRI Analysis of Contrast Agent Transport Across the Blood-Perilymph Barrier and Intrastrial Fluid-Blood Barrier in the Mouse Inner Ear.

摘要: HYPOTHESIS Effective paramagnetic contrast agent for the penetration of perilymphatic spaces scala tympani, vestibuli, and media mouse inner ear can be determined using intravenous injection various gadolinium (Gd) complexes ultra-high-field magnetic resonance imaging (MRI) at 9.4 Tesla. BACKGROUND A number agents have been explored in experimental high-field MRI to determine most effective Gd complex ideal signal-to-noise ratio maximal visualization vivo mammalian analyzing temporal spatial parameters involved drug blood-perilymph barrier intrastrial fluid-blood model MRI. METHODS Gadoteric acid (Dotarem), Gadobutrol (Gadovist), Gadodiamide (Omniscan), Gadopent (Magnevist), Mangafodipir (Teslascan) were administered intravenously tail vein 60 Balb/C mice. High-resolution T1 images acquired with a horizontal T Agilent magnet after injection. Signal intensity was used as metric delivery endolymphatic spaces. RESULTS ANOVA analysis area under curve enhancement perilymph revealed significant difference (p < 0.05) scalae uptake different (F (3,25) = 3.54, p = 0.029). The Dotarem found compound terms rapid, morphological temporal, distribution space ear. CONCLUSION (Dotarem) demonstrated efficacy enhanced labyrinthine mouse, including tympani vestibuli cochlea, semicircular canals vestibular apparatus. These findings may inform clinical application compounds patients fluid disorders vertigo.