Comparative study of seven commercial kits for human dna extraction from urine samples suitable for dna biomarker-based public health studies

摘要: Human genomic DNA extracted from urine could be an interesting tool for large-scale public health studies involving characterization of genetic variations or biomarkers as a result the simple and noninvasive collection method. These studies, many samples, require rapid, easy, standardized extraction protocol. Moreover, practicability, there is necessity to collect at moment different first void store it appropriately until analysis. The present study compared seven commercial kits select most appropriate urinary human procedure epidemiological studies. yield has been determined using quantification methods: two classical, i.e., NanoDrop PicoGreen, species-specific real-time quantitative (q)PCR assays, contains, besides human, microbial also, which largely contributes total yield. In addition, giving good were also tested presence PCR inhibitors. Further comparisons performed regarding sampling time storage conditions. Finally, proof-of-concept, important gene related smoking genotyped developed tools. We one well-performing kit suitable molecular diagnostic qPCR-based assays targeting variations, applicable successful genotyping was possible stored -20°C several months, acceptable obtained collected moments during day, particularly