Biochip for fluorescence analysis of individual transporters
作者： Matthias Pirsch , Stefan Hummel
DOI:
关键词: Biochip 、 Nanotechnology 、 Chemistry 、 Optoelectronics 、 Substrate (printing) 、 Fluorescence 、 Transporter 、 Membrane
摘要: The invention relates to a biochip ( 1 ) for optical measurement of the properties individual transporter systems 50 ). In order measure molecules with great accuracy and at high throughput, is proposed, which essentially consists transparent carrier 10 multiple depressions 30 open top, whereby configured in such manner that its openings 31 can be covered by membrane 40 ), thus closed chambers are formed, transport substrate 60 into way detected.
lens.org 参考文章(19)
Anthony G. Frutos, Joydeep Lahiri, Ye Fang, Substrates with stable surface chemistry for biological membrane arrays and methods for fabricating thereof ,(2002)
Valerie Lacarriere, Alexander M. Efremov, John C. Mauro, Joydeep Lahiri, Srikanth Raghavan, Yulong Hong, Alain R.E. Carre, Fang Lai, Brian L. Webb, Ye Fang, Membrane arrays and methods of manufacture ,(2004)
David P. Stumbo, Douglas N. Modlin, Light detection device with means for tracking samples sites ,(2001)
Gönül Veliçelebi, James A. Dykens, Soumitra S. Ghosh, Compositions and methods for assaying subcellular conditions and processes using energy transfer ,(1999)
Carlos Gitler, Itzhak Yuli, Biosensors including lipid bilayer doped with ion channels anchored to a recording electrode by bridging molecules ,(1991)
Frances S. Ligler, Thomas L. Fare, Receptor-based sensor ,(1990)
Walter R. Vandaveer, Ingrid Fritsch, Charles Sherman Henry, Nicole Bratcher, Benjamin P. Bowen, Multilayer microcavity devices and methods ,(2001)
Steven A. Sundberg, Jeffrey A. Wolk, Calvin Y. H. Chow, Joseph E. Kercso, Andrew W. Toth, J. Wallace Parce, High throughput microfluidic systems and methods ,(2002)
Simon D. Ogier, Richard J. Bushby, Yaling Cheng, Stephen D. Evans, Stuart W. Evans, A. Toby A. Jenkins, Peter F. Knowles, Robert E. Miles, Suspended Planar Phospholipid Bilayers on Micromachined Supports Langmuir. ,vol. 16, pp. 5696- 5701 ,(2000) , 10.1021/LA991367O
K.T. Samiee, J.M. Moran-Mirabal, Y.K. Cheung, H.G. Craighead, Zero mode waveguides for single-molecule spectroscopy on lipid membranes. Biophysical Journal. ,vol. 90, pp. 3288- 3299 ,(2006) , 10.1529/BIOPHYSJ.105.072819