Metabolically engineered E. coli for enhanced production of oxaloacetate-derived biochemicals
作者： Ravi R. Gokarn , Mark A. Eiteman , Elliot Altman
DOI:
关键词: Biochemistry 、 Enzyme 、 Citrate synthase 、 Pyruvate carboxylase 、 Lysine 、 Biology 、 Metabolic engineering 、 Carbon flow
摘要: Metabolic engineering is used to increase the carbon flow toward oxaloacetate enhance production of bulk biochemicals, such as lysine and succinate, in bacterial fermentations. Carbon redirected by genetically cells overexpress enzyme pyruvate carboxylase.
google.de 参考文章(107)
R O'Brien, D T Chuang, B L Taylor, M F Utter, Novel enzymic machinery for the metabolism of oxalacetate, phosphoenolpyruvate, and pyruvate in Pseudomonas citronellolis. Journal of Biological Chemistry. ,vol. 252, pp. 1257- 1263 ,(1977) , 10.1016/S0021-9258(17)40649-1
Martin D. Diesterhaft, Ernst Freese, Role of Pyruvate Carboxylase, Phosphoenolpyruvate Carboxykinase, and Malic Enzyme during Growth and Sporulation of Bacillus subtilis Journal of Biological Chemistry. ,vol. 248, pp. 6062- 6070 ,(1973) , 10.1016/S0021-9258(19)43509-6
Katsura IZUI, Masaaki TAGUCHI, Minoru MORIKAWA, Hirohiko KATSUKI, Regulation of Escherichia coli Phosphoenolpyruvate Carboxylase by Multiple Effectors In Vivo. II. Kinetic Studies with a Reaction System Containing Physiological Concentrations of Ligands Journal of Biochemistry. ,vol. 90, pp. 1321- 1331 ,(1981) , 10.1093/OXFORDJOURNALS.JBCHEM.A133597
Mark Schena, Didier Picard, Keith R. Yamamoto, Vectors for constitutive and inducible gene expression in yeast. Methods in Enzymology. ,vol. 194, pp. 389- 398 ,(1991) , 10.1016/0076-6879(91)94029-C
Toshinao Tsunoda, Shin-Ichiro Ôtsuka, Isamu Shiio, GLUTAMIC ACID FORMATION FROM GLUCOSE BY BACTERIA:III. ON THE PATHWAY OF PYRUVATE FORMATION IN BREVIBACTERIUM FLAVUM NO. 2247 Journal of Biochemistry. ,vol. 47, pp. 414- 421 ,(1960)
Christopher J. Lemme, Rathin Datta, Process for the production of succinic acid by anaerobic fermentation ,(1987)
Noushin Irani, Joop Van Der Heuvel, Roland Wagner, Manfred Wirth, Method of improving the primary energy metabolism of mammalian cell lines ,(2000)
F Lim, C P Morris, F Occhiodoro, J C Wallace, Sequence and domain structure of yeast pyruvate carboxylase Journal of Biological Chemistry. ,vol. 263, pp. 11493- 11497 ,(1988) , 10.1016/S0021-9258(18)37984-5
Reinhold Seipenbusch, Process for the production of L-malic acid ,(1985)
Raymond L Rodriguez, Robert C Tait, None, Recombinant DNA Techniques: An Introduction ,(1983)