Resazurin assay of radiation response in cultured cells.
作者： S Anoopkumar-Dukie , J B Carey , T Conere , E O'Sullivan , F N van Pelt
DOI: 10.1259/BJR/54004230
关键词: Resazurin 、 Oxazines 、 DNA synthesis 、 Pathology 、 Bromodeoxyuridine 、 Tumor Stem Cell Assay 、 Cell culture 、 Oxygen enhancement ratio 、 Radiation response 、 Chemistry 、 Chromatography
摘要: We describe use of resazurin reduction for measurement cell response to irradiation as a simple and non-destructive assay that complements the conventional colony forming can readily be applied both adherent non-adherent cultures. The method yields data comparable with well DNA synthesis (BrdU incorporation), giving an OER (oxygen enhancement ratio) 2.5 at 60% isoeffect level versus 3.1 assay. Intraday interday precisions were 4.1% 5.2%, respectively.
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