L-Serine Treatment May Improve Neurorestoration of Rats after Permanent Focal Cerebral Ischemia Potentially Through Improvement of Neurorepair

摘要: The present study was conducted to clarify whether treatment with L-serine can improve the brain repair and neurorestoration of rats after permanent middle cerebral artery occlusion (pMCAO). After pMCAO, neurological functions, lesion volume, cortical injury were determined. GDNF, NGF, NCAM L1, tenascin-C, Nogo-A levels measured. Proliferation differentiation neural stem cells (NSCs) proliferation microvessels in ischemic boundary zone cortex evaluated. Treatment (168 mg/kg body weight, i.p.) began 3 h pMCAO repeated every 12 for 7 days or until end experiment. L-Serine treatment: 1) reduced volume neuronal loss; 2) improved recovery functions; 3) elevated expression nerve growth-related factors; 4) facilitated endogenous NSCs activated increased number new-born neurons. 5) D-cycloserine, an inhibitor serine hydroxymethyltransferase, blunted effects on NSC proliferation, differentiation, microvascular proliferation. In conclusions, reduce facilitate which is partly associated improvement microvessels, reconstruction neurovascular units resultant neurorepair. are mediated by action as a substrate production one-carbon groups used purine pyrimidine synthesis modulation some factors.