Thiolated Salmonella antibody immobilization onto the gold surface of piezoelectric quartz crystal1This paper was presented at the fifth World Congress on Biosensors, Berlin, Germany, 3–5 June 1998.1
作者： In-Seon Park , Namsoo Kim
DOI: 10.1016/S0956-5663(98)00067-0
关键词: Quartz crystal microbalance 、 Analytical chemistry 、 Suspension (vehicle) 、 Biosensor 、 Reagent 、 Electrode 、 Materials science 、 Nuclear chemistry 、 Crystallization 、 Quartz 、 Crystal 、 Biotechnology 、 Biophysics 、 Electrochemistry 、 Biomedical engineering 、 General Medicine
摘要: An improved antibody-coated sensor system based on quartz crystal microbalance analysis Salmonella spp. was developed making use of thiolated antibody immobilization onto one gold electrode the piezoelectric surface. The best results in sensitivity and stability were obtained with thin layer a thiol-cleavable, heterobifunctional cross-linker, sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP). long bridge this reagent could function as spacer, facilitating antibody-Salmonella interaction electrode. After addition S. typhimurium suspension into reaction cell 0.1 M sodium phosphate buffer, pH 7.2, resonant frequency decreased conspicuously. time required for maximum shift about 30-90 min. Sensor response observed microbial suspensions ranging from 9.9 x 10(5) to 1.8 10(8) CFU/ml.
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