Expression in BALB/c and C57BL/6 mice of Rt6-1 and Rt6-2 ADP-ribosyltransferases that differ in enzymatic activity: C57BL/6 Rt6-1 is a natural transferase knockout.

摘要: Several proteins with NAD+:arginine ADP-ribosyltransferase (ART) activity are expressed in T cells and affect their function. Rat that express the ART designated RT6 determinants of expression autoimmune diabetes. In mouse, a 35-kDa ecto-ART modulates proliferation functional CTL. Here we report on mouse ARTs Rt6-1 Rt6-2 BALB/c C57BL/6 mice. mRNAs for were found spleen, thymus, intestinal tissue both strains, but mRNA mice was detected only at low levels. cDNAs from strains cloned sequenced. Predicted amino acid sequences identical there an in-frame stop codon sequence not present Recombinant COS1 exhibited documented to be glycosylphosphatidylinositol-linked membrane proteins. COS-1 transfected cDNA construct truncated protein consistent size predicted by presence codon. This approximately 21-kDa appeared glycosylphosphatidylinositol linked cell surface lacked activity. therefore appears naturally occurring knockout. The lymphoid tissues suggests these may regulate immune system functions. Expression or another redundant compensate lack enzymatically active