Time-related dynamics of variation in core clock gene expression levels in tissues relevant to the immune system.

摘要: Immune parameters show rhythmic changes with a 24-h periodicity driven by an internal circadian timing system that relies on clock genes (CGs). CGs form interlocked transcription-translation feedback loops to generate and maintain mRNA protein oscillations. In this study we evaluate compare the profiles dynamics of variation CG expression in peripheral blood, two lymphoid tissues mice. Expression levels seven recognized key (mBmal1, mClock, mPer1, mPer2, mCry1, mCry2, Rev-erbalpha) were evaluated quantitative RT- PCR spleen, thymus blood C57BL/6 male mice housed 12-h light (L)-dark (D) cycle sacrificed every 4 h for 24 (3-4 mice/time point). We found statistically significant time-effect spleen (S), (T) (B) original values level mBmal1 mClock (T, B), mPer1 (S, mPer2 mCry1 mCry2 mRev-Erbalpha T, B) fractional calculated between single time-point value (B), (T), (S). A rhythm was validated five (mClock, mRev-Erbalpha), four three mRev-Erbalpha). The acrophases mBmal1, very similar advanced several hours compared tissues, whereas phases coincident all tissues. conclusion, central mouse different sequences activation gene blood. These differences may underlie compartmental pattern web functioning immune system.