Characteristics of cloned cells of mixed müllerian tumor of the human uterus carcinoma cells showing myogenic differentiation in vitro

摘要: Background. To elucidate the relationship between epithelial and mesenchymal elements of malignant mixed Mullerian tumors (MMMT), authors examined biologic properties two clones different cell types (designated as FU-MMT-2-C1 FU-MMT-2-S1) established from a uterine MMMT line (FU-MMT-2), which they previously have reported. Methods Results. By morphologic immuno-cytochemical analyses, exhibited features adenocarcinoma cells, whereas FU-MMT-2-S1 showed characteristics sarcoma cells with myogenic differentiation. Some at confluence differentiated spontaneously into in vitro. In addition, transitional-type sarcomatous were observed areas differentiation by light electron microscopic study. Ultrastructurally, represented biphasic consisting features, proved to coexpress epithelial, mesenchymal, muscle markers double immunoenzymatic staining. However, no differentiations apparent clone FU-MMT-2-S1. produced tumor nude mice, histologic study mixture that resembled original tumor. Cytogenetic studies demonstrated these monoclonal origin because presence common karyotypic abnormalities both cells. amplified (approximately fourfold eightfold) c-myc oncogene was found cloned cells. Conclusions. The current results strongly support theory single suggest originated primitive capable differentiating or elements.