Rhodococcus lactonase with organophosphate hydrolase (OPH) activity and His 6 -tagged OPH with lactonase activity: evolutionary proximity of the enzymes and new possibilities in their application
作者： Mariia Sirotkina , Elena N. Efremenko
DOI: 10.1007/S00253-013-5233-Y
关键词: Rhodococcus 、 Biodegradation 、 Enzyme 、 Paraoxon 、 Lactonase 、 Chemistry 、 Bacteria 、 Biochemistry 、 Hydrolysis 、 Microorganism
摘要: Decontamination of soils with complex pollution using natural strains microorganisms is a matter great importance. Here we report that oil-oxidizing bacteria Rhodococcus erythropolis AC-1514D and ruber AC-1513D can degrade various organophosphorous pesticides (OP). Cell-mediated degradation five different OP apparently associated the presence N-acylhomoserine lactonase, which pronouncedly similar (46–50 %) to well-known enzyme organophosphate hydrolase (OPH), hydrolysis catalyst for wide variety compounds. Additionally, demonstrated high lactonase activity hexahistidine-tagged (His6-OPH) respect lactones, determined catalytic constants His6-OPH towards these These experimental data theoretical analysis confirmed hypothesis about evolutionary proximity OPH lactonases. Using cells, carried out effective simultaneous biodegradation pesticide paraoxon (88 mg/kg) oil hydrocarbon hexadecane (6.3 g/kg) in soil. Furthermore, discovered offers new possibilities developing an efficient strategy combating resistant populations Gram-negative bacterial cells.
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