Hydrolysis of organophosphorus pesticides in soil: New opportunities with ecocompatible immobilized His6-OPH
作者： M. Sirotkina , I. Lyagin , E. Efremenko
DOI: 10.1016/J.IBIOD.2011.12.004
关键词: Bioremediation 、 Straw 、 Soil type 、 Parathion 、 Environmental chemistry 、 Paraoxon 、 Soil test 、 Diazinon 、 Pesticide 、 Chemistry
摘要: Abstract Immobilized biocatalyst developed on the basis of hexahistidine-tagged organophosphorus hydrolase (His6-OPH) was tried for bioremediation various soil samples heavily contaminated by pesticides. Various cellulose-containing carriers being agricultural wastes were applied His6-OPH immobilization enabling stabilization enzyme and its retention in pesticides destruction zone. Straw-based immobilized enzymatic preparation ensured decomposition 630 mg Paraoxon or 850 mg Diazinon 185 mg Parathion per kg less than 10 days; meanwhile, half-life active sand estimated as 130 days. allowed to hydrolyze pesticide case reintroduction into samples. The influence type kinetics revealed.
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