Biosynthesis of the Polysaccharide of Micrococcus lysodeikticus Cell Walls I. CHARACTERIZATION OF AN IN VITRO SYSTEM FOR POLYSACCHARIDE BIOSYNTHESIS
作者： Robert L. Page , John S. Anderson
DOI: 10.1016/S0021-9258(19)45452-5
关键词: Polysaccharide 、 Biosynthesis 、 Substrate (chemistry) 、 Enzyme 、 Biochemistry 、 Uridine 、 Residue (chemistry) 、 Chemistry 、 Cell wall 、 Magnesium ion
摘要: Abstract The particulate enzyme fraction obtained from Micrococcus lysodeikticus catalyzed the incorporation of d-[14C]glucose uridine diphospho-d-[14C]glucose into a polymer which resembled d-glucose- and N-acetyl-d-mannosaminuronic acid-containing polysaccharide cell walls this organism. In vitro biosynthesis depended upon presence diphospho-N-acetylhexosaminuronic acid isolated M. diphospho-N-acetyl-d-glucosamine was stimulated by heat-stable factor soluble extracts. reaction proceeded optimally at pH 8.2 20 mm magnesium ion concentration. after lag period 15 to min. Preincubation components with before addition diphospho-d-glucose eliminated period. [14C]N-Acetylhexosaminuronic residues were incorporated [14C]uridine (labeled throughout molecule) in an amount approximately equimolar d-glucose dependent diphospho-d-glucose. However, when diphospho-N-[14C]acetyl-d-glucosamine labeled substrate, N-[14C]acetyl-d-glucosamine only extent 1 residue or less for each d-glucose.
sci-hub.st 参考文章(44)
R. C. Hughes, The cell wall of Bacillus licheniformis N.C.T.C. 6346. Linkage between the teichuronic acid and mucopeptide components Biochemical Journal. ,vol. 117, pp. 431- 439 ,(1970) , 10.1042/BJ1170431
Sudhamoy Ghosh, Saul Roseman, THE SIALIC ACIDS. IV. N-ACYL--D-GLUCOSAMINE 6-PHOSPHATE 2-EPIMERASE. Journal of Biological Chemistry. ,vol. 240, pp. 1525- 1530 ,(1965) , 10.1016/S0021-9258(18)97467-3
Meir Lahav, T.H. Chiu, W.J. Lennarz, Studies on the Biosynthesis of Mannan in Micrococcus lysodeikticus: II. THE ENZYMATIC SYNTHESIS OF MANNOSYL-1-PHOSPHORYL-UNDECAPRENOL Journal of Biological Chemistry. ,vol. 244, pp. 5890- 5898 ,(1969) , 10.1016/S0021-9258(18)63557-4
Walther Katz, Michio Matsuhashi, Carl P. Dietrich, Jack L. Strominger, Biosynthesis of the Peptidoglycan of Bacterial Cell Walls Journal of Biological Chemistry. ,vol. 242, pp. 3207- 3217 ,(1967) , 10.1016/S0021-9258(18)95951-X
M Scher, W J Lennarz, Studies on the Biosynthesis of Mannan in Micrococcus lysodeikticus I. CHARACTERIZATION OF MANNAN-14C FORMED ENZYMATICALLY FROM MANNOSYL-1-PHOSPHORYL-UNDECAPRENOL Journal of Biological Chemistry. ,vol. 244, pp. 2777- 2789 ,(1969) , 10.1016/S0021-9258(18)83463-9
John S. Anderson, Pauline M. Meadow, Mary A. Haskin, Jack L. Strominger, Biosynthesis of the peptidoglycan of bacterial cell walls Archives of Biochemistry and Biophysics. ,vol. 116, pp. 487- 515 ,(1966) , 10.1016/0003-9861(66)90056-7
John S. Anderson, Michio Matsuhashi, Mary A. Haskin, Jack L. Strominger, Biosynthesis of the Peptidoglycan of Bacterial Cell Walls Journal of Biological Chemistry. ,vol. 242, pp. 3180- 3190 ,(1967) , 10.1016/S0021-9258(18)95949-1
P Biely, R W Jeanloz, The Isolation and Structure Identification of Uridine 5-(2-Acetamido-2-deoxy-α-D-glucopyranosyluronic Acid Pyrophosphate) from Micrococcus lysodeikticus Cells Journal of Biological Chemistry. ,vol. 244, pp. 4929- 4935 ,(1969) , 10.1016/S0021-9258(18)94292-4
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Spencer Rosenthal, Nathan Sharon, The use of Sephadex G-25 for the isolation of nucleotide sugar derivatives from Micrococcus lysodeikticus Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides. ,vol. 83, pp. 378- 380 ,(1964) , 10.1016/0926-6526(64)90025-4